BFE VFE Bio Kit Bioaerosol Sampling System

In Accordance with: EN 14683 • ASTM F2100 • ASTM F2101 • EN ISO 9237

BFE (Bacterial Filtration Efficiency) and VFE (Viral Filtration Efficiency) Bio Kit Bioaerosol Sampling System, allows to be in compliance with all the requests for BFE % efficiency test (Bacterial filtration efficiency), VFE Viral Filtration Efficiency test and breathability test (Pa/cm²) for medical face mask materials according to the EN14683 as well as according to ASTM 2100 and ASTM 2101. BFE Bio kit is also done for EN ISO 9237 for measuring the permeability of fabrics to air and it is applicable to most types of fabrics, including industrial fabrics for technical purposes, nonwovens and made-up textile articles that are permeable to air.

BFE Bio Kit Bioaerosol SAmpling System

Particle Nebulyzer


Aerosol Chamber


6 Stage Impactor


Extraction Condenser


Electronic Flow Control Sampler

How to proceed the Bacterial Filtration Efficiency (BFE) and VFE Viral Filtration Efficiency Testing

EN 14683 (Annex B) – ASTM 2101

  1. Fill in the aerosol generator with the bacteria suspension to nebulize;
  2. Set up the multi stage cascade impactor and connect the sampling pipe;
  3. Connect the atomization chamber to the impactor;
  4. Connect the condenser at the impactor output suction line;
  5. Connect the output of the condenser to the sampler, setting a 28,3 l/min flow;
Laboratory Analysis

Bioaerosol Sampling System


  • Microprocessor data management;
  • High precision in the measurement of flow and volume;
  • HEPA filter at the exhaust end of the vacuum pump;
  • Download report USB;
  • Flowmeter and Differential Pressure reader integrated;
  • Multiple repetition of tests with aid of configured functions.

Breathability and Pearmeability (differential pressure)

EN14683 (Annex C) • ASTM F2100 • EN ISO 9237

The determination of differential pressure is performed under a specific constant flow condition and taking in account temperature and humidity during the test.

Unless otherwise specified, the breathability test (EN 14683 Annex C – ASTM 2100) shall be performed with the air flow direction from the inside of the mask to the outside of the mask.

Particle sampled holders to recover the sample, made of material as requested from the standard, are delivered.

The BFE VFE Bio Kit is suitable for measuring also the permeability of fabrics to air (EN ISO 9237). 

  • Sample holder 80 mm diameter made in PTFE – BFE test (EN 14683 Annex B; ASTM F2101)
  • Sample holders 25 mm diameter Stainless steel AISI316 – Breathability (EN 14683 Annex C; ASTM F2100)
  • Sample holder 5 cm2 – 20 cm2 – 50 cm2 – 100 cm2 – Permeability ( EN ISO 9237 )
BFE Bio Kit Breathing Efficiency Test TCR Tecora

6 Stage Andersen Cascade Impactor

Principle of Operation

From the standpoint of respiratory deposition, the human respiratory system acts as an aerodynamic airborne particle classifier.

6 Stage Andersen Cascade Impactor - TCR Tecora

The multi stage impactor, based on the particle inertial impaction principle, simulates the human respiratory tract (extra thoracic, tracheobronchial, alveolar).

The micro holes in each of the 6 impactor stages act as nozzles that, in function of the hole diameter and impaction distance, lets the caption of a dimensional range of particles, with a defined cutoff diameter and a characteristic efficiency impaction curve.

The special design of the multi stage impactor assures the collection of size-segregated particles that are suspended in the sample air.

Multistage Cascade Impactor Principle of Operation

BFE VFE Bio Kit – Bioaerosol Sampling System


In accordance with:
EN 14683 EN 9237ASTM F2101

Bravo Aero Particle Nebulizer BFE Bio Kit TCR Tecora

Particle Nebulyzer

COD: AC99-120-0000SP

  • Aerosol generator compatible with all type of liquids, suspensions and solutions;
  • Integrated pump (no compressed air required);
  • Adjustable Nebulyzing and dilution air (dry) flow;
6 Stage Andersen Cascade Impactor BFE Bio Kit TCR Tecora

6 Stage Andersen Cascade Impactor

COD: AC99-120-0002SP

  • Functioning principle: Inertial impaction;
  • Required Flow: 28,3 l/min (1 CFM);
  • Direct sampling on 90 mm petri plates;
  • Made of corrosion resistant material
Aerosol Chamber BFE Bio Kit TCR Tecora

Aerosol Chamber

COD. AC99-120-0001SP

  • Pyrex tempered glass, dimensions 600mm x 80mm Ø;
  • Upper PTFE flange (HEPA filter included);
  • Lower PTFE flange (included: BFE sample holder)
  • Connections for: Aerosol Generator; 6 stages impactor;  n° 4 side connectors (optical particle counter option)
Extraction Condenser BFE Bio Kit TCR Tecora

Extraction Condenser

COD: AC99-120-0003SP

  • PYREX glass Condenser;
  • PYREX glass Condensation vessel.
Bravo Bio Electronic Flow Control Sampler Bfe Bio Kit TCR Tecora

Electronic Flow Control Sampler

COD: AA99-000-0030SP [Bravo Basic H]
COD: AA99-000-0740SP [Bravo X BIO] (differential pressure)

  • Automatic flow regulation;
  • Flow range: 0.5 to 70 l/min;
  • Available models: Basic H or X-BIO;
  • HEPA filter (pump exhaust) included.

Download the Datasheet

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